![]() In China, Acanthopanax gracilistylus wine, which is made of Acanthopanacis cortex and other herbs soaked in liquor, is popularly used as a health supplement product to treat rheumatic arthritis because of its clinical effects. It is commonly used to expel wind-dampness, tonify liver and kidneys, and strengthen muscles and bones, in accordance with Chinese medicine theory ( State Pharmacopoeia Committee, 2010). Hu ( State Pharmacopoeia Committee, 2010). In conclusion, the ITS2 barcode effectively identifies Acanthopanacis cortex, and DNA barcoding is a convenient tool for medicine market supervision.Īcanthopanacis cortex, a traditional Chinese medicine known as “wujiapi,” is derived from the dried root barks of Eleutherococcus nodiflorus (Dunn) S.Y. ![]() A secondary structure of ITS2 provided another dimensionality to identify species. The NJ tree indicated that the Acanthopanacis cortex samples clustered into one clade, which can be distinguished clearly from the adulterants of this herb. Three stable SNPs in ITS2 can be used to distinguish Acanthopanacis cortex and its closely related species. ![]() giraldii was 0.0221, which was larger than the maximum intraspecific distance of E. The minimum interspecific distance between E. According to the kimura-2-parameter model, the intraspecific distance of Eleutherococcus nodiflorus ITS2 sequences ranged from 0 to 0.0132. The nearest distance, single-nucleotide polymorphisms (SNPs), and neighbor-joining (NJ) tree methods were used to evaluate the identification ability of the ITS2 barcode. In this study, we used the internal transcribed spacer 2 (ITS2) barcode to identify 69 samples belonging to six species, including Acanthopanacis cortex and its adulterants. Considering some historical and geographical factors, Acanthopanacis cortex is easily confused with other herbs in medicine markets, thereby causing potential safety issues. 2Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaĪcanthopanacis cortex has been used in clinical applications for a long time.1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.Sha Zhao 1, Xiaochen Chen 1, Jingyuan Song 1, Xiaohui Pang 1 * and Shilin Chen 2 *
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